Immune functionalization of outer membrane proteins (OMPs) for enteropathogenic E.coli in vivo

This study aimed to reveal the susceptibility of Outer Membrane Proteins (OMPs) Enteropathogenic E.coli (EPEC) after linking it an immunosuppressant (from natural sources)to stimulate immune system laboratory animals as a primitive attempt initiate manufacture vaccine against above bacteria and reduce fatalities that occur in children under age five when injuredRelying on some common methods, outer membrane proteins were separated from EPEC amount 200 mg / 20 L culture medium.The molecular weight purity this protein detected using Sodium Dodecyl Sulfate-Polyacrylamide gel ( SDS-PAGE), OMP appeared form ladder containing successive bundles consisting (9-13) polypeptides, whose ranges between (15-125) kDa.The ability bacteria, its (OMPs),the helper (olive oil), foreign complex with induce systemic immunity through changes antibody titer averages studied.A significant increase antibodies was observed treated mice (IgG IgA) compared positive control group those before treatment.